Identification of Alkaloids from Hippeastrum aulicum (Ker Gawl.) Herb. (Amaryllidaceae) Using CGC-MS and Ambient Ionization Mass Spectrometry (PS‑MS and LS-MS)

Ms Imaging and Ambient Ionization-ms

Identification of oxidized phospholipids by electrospray ionization mass spectrometry and LC-MS using a QQLIT instrument.

Phospholipids are complex and varied biomolecules that are susceptible to lipid peroxidation after attack by free radicals or electrophilic oxidants and can yield a large number of different oxidation products. There are many available methods for detecting phospholipid oxidation products, but also various limitations and problems. Electrospray ionization mass spectrometry allows the simultaneo...

Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of isopimarane diterpenes from Velloziaceae

This is the peer reviewed version of the following article: a Cunha Pinto, A., Vessecchi, R., da Silva, C. G., Amorim, A. C. L., dos Santos Júnior, H. M., Rezende, M. J. C., Gates, P. J., Rezende, C. M., and Lopes, N. P. (2016) Electrospray ionization tandem mass spectrometry analysis of isopimarane diterpenes from Velloziaceae. Rapid Commun. Mass Spectrom., 30: 61–68., which has been published...

Identification of mammalian cell lines using MALDI-TOF and LC-ESI-MS/MS mass spectrometry.

Direct mass spectrometric analysis of complex biological samples is becoming an increasingly useful technique in the field of proteomics. Matrix-assisted laser desorption/ionization mass spectroscopy (MALDI-MS) is a rapid and sensitive analytical tool well suited for obtaining molecular weights of peptides and proteins from complex samples. Here, a fast and simple approach to cellular protein p...

Quantification of Signaling Lipids by Nano-Electrospray Ionization Tandem Mass Spectrometry (Nano-ESI MS/MS)

Lipids, such as phosphoinositides (PIPs) and diacylglycerol (DAG), are important signaling intermediates involved in cellular processes such as T cell receptor (TCR)-mediated signal transduction. Here we report identification and quantification of PIP, PIP2 and DAG from crude lipid extracts. Capitalizing on the different extraction properties of PIPs and DAGs allowed us to efficiently recover b...